Root and crown rot pathogens found on dry beans grown in Mozambique

Dry edible beans are a vital food source in Mozambique, East Africa—one that alleviates hunger and malnutrition adds value to the economy. In recent years, root/crown rot (RCR) pathogens have emerged as limiting constraints dry bean production. Not much has been characterized concerning causal agents of RCR Mozambique. The purpose this study was identify primary pathogen(s) associated with samples collected at breeder nursery sites farmer fields Mozambique using molecular sequencing culture-based methods. Sequencing revealed, not surprisingly, an increased diversity fungal/oomycete operational taxonomic units when compared methods oof diversity. Species Fusarium, mainly F. oxysporum, were dominant taxa detected through ITS rDNA region partial EF-1α gene. Collectively, 333 fungi and/or Oomycetes isolated culture during 2014–2015 growing seasons tested for pathogenicity on healthy seedlings. Fusarium species identified by both morphological characters. At least 60% isolates inoculated common recognized potentially pathogenic. From isolation frequency testing, oxysporum related play important role complex. We found similar results from grown two main bean-growing regions These findings will allow breeders screen resistance greenhouse plants well within field cultivars.